Genome wide association study and differential gene expression of Drosophila melanogaster under heat and cold stress
TS O’Leary
1 MA plots
1.1 Fold-change cutoff 2
Cold v Ctrl MA plot
Hot v Ctrl MA plot
1.2 No fold-change cutoff
Cold v Ctrl MA plot
Hot v Ctrl MA plot
1.3 No fold-change cutoff (fdr < 0.01)
Cold v Ctrl MA plot
Hot v Ctrl MA plot
2 Top 50 differentially expressed genes
2.1 Heat shock
| gene | hot_fc | log2FoldChange.hot | padj.hot |
|---|---|---|---|
| CG3339 | down | -5.155 | 1.097e-27 |
| l(2)08717 | down | -2.3 | 4.051e-25 |
| pip | down | -3.434 | 5.188e-24 |
| stan | down | -2.592 | 2.063e-23 |
| Dhc36C | down | -2.724 | 1.51e-22 |
| ft | down | -3.361 | 4.602e-22 |
| CG17075 | down | -2.455 | 5.379e-22 |
| CG1801 | down | -3.692 | 2.458e-21 |
| CG9492 | down | -4.122 | 2.881e-20 |
| Dhc93AB | down | -3.104 | 1.029e-19 |
| Dscam1 | down | -1.795 | 1.792e-19 |
| CG42534 | down | -4.148 | 4.16e-19 |
| CG6125 | down | -2.153 | 7.197e-19 |
| CG1688 | down | -3.061 | 8.789e-19 |
| btv | down | -4.624 | 1.002e-18 |
| Sb | down | -4.59 | 1.126e-18 |
| CG43980 | down | -4.82 | 1.34e-18 |
| Muc14A | down | -2.612 | 1.372e-18 |
| hiw | down | -1.521 | 1.414e-18 |
| CG3348 | down | -3.219 | 1.822e-18 |
| Nos | down | -3.834 | 2.877e-18 |
| CG30263 | down | -3.978 | 4.212e-18 |
| jv | down | -2.334 | 4.212e-18 |
| conv | down | -4.136 | 4.94e-18 |
| NT1 | down | -6.652 | 4.94e-18 |
| Dop1R1 | down | -2.643 | 6.474e-18 |
| Shawl | down | -3.459 | 8.688e-18 |
| CG8745 | down | -2.145 | 1.314e-17 |
| CG14304 | down | -4.486 | 1.542e-17 |
| CG31465 | down | -6.373 | 2.078e-17 |
| Ugt86Di | down | -5.074 | 4.038e-17 |
| CR44327 | down | -2.536 | 4.654e-17 |
| salr | down | -3.028 | 4.671e-17 |
| nompC | down | -3.89 | 6.068e-17 |
| SIFaR | down | -4.022 | 6.357e-17 |
| Nlg4 | down | -2.488 | 8.891e-17 |
| CG15894 | down | -2.427 | 1.946e-16 |
| Cda5 | down | -1.12 | 2.097e-16 |
| CG4744 | down | -6.327 | 2.705e-16 |
| eag | down | -1.729 | 2.931e-16 |
| CG30272 | down | -3.39 | 3.009e-16 |
| Ac78C | down | -2.352 | 3.672e-16 |
| CG45105 | down | -3.336 | 4.007e-16 |
| Rab26 | down | -2.214 | 4.049e-16 |
| CG12950 | down | -2.918 | 4.628e-16 |
| CG32791 | down | -1.702 | 5.419e-16 |
| TkR99D | down | -3.825 | 8.6e-16 |
| Ddr | down | -3.329 | 9.219e-16 |
| GABA-B-R1 | down | -2.256 | 1.09e-15 |
| Sytbeta | down | -3.292 | 1.449e-15 |
| Hsp68 | up | 6.888 | 0 |
| DnaJ-1 | up | 3.727 | 2.219e-143 |
| Hsp70Bb | up | 7.003 | 2.219e-143 |
| stv | up | 4.471 | 2.373e-104 |
| Hsp26 | up | 4.279 | 5.676e-72 |
| Hsp67Bc | up | 4.785 | 2.957e-71 |
| Hsp27 | up | 3.532 | 4.06e-62 |
| Hsp83 | up | 2.106 | 6.281e-58 |
| Hsp23 | up | 6.872 | 8.789e-53 |
| Nop17l | up | 1.614 | 7.563e-52 |
| CG6770 | up | 1.78 | 5.402e-48 |
| Hop | up | 1.305 | 1.268e-43 |
| CG42240 | up | 1.131 | 1.65e-41 |
| Hsc70Cb | up | 1.271 | 5.313e-38 |
| Jafrac1 | up | 0.8308 | 7.251e-38 |
| CG16721 | up | 1.484 | 1.699e-36 |
| CG1416 | up | 1.24 | 1.833e-34 |
| CG5290 | up | 1.882 | 8.359e-34 |
| Droj2 | up | 1.165 | 9.102e-33 |
| Hsc70-3 | up | 1.522 | 2.984e-32 |
| Lsd-2 | up | 1.004 | 2.845e-30 |
| alphagamma-element:CR32865 | up | 6.317 | 1.507e-29 |
| sra | up | 1.258 | 9.836e-29 |
| CG10600 | up | 1.189 | 1.267e-28 |
| Mlf | up | 1.019 | 1.689e-28 |
| Capr | up | 0.9256 | 6.875e-28 |
| mim | up | 1.18 | 1.125e-26 |
| Hsp70Bc | up | 5.778 | 5.299e-26 |
| CG10077 | up | 1.398 | 5.714e-26 |
| Pgi | up | 0.9688 | 3e-25 |
| ref(2)P | up | 1.398 | 7.851e-24 |
| CG7033 | up | 1.009 | 4.144e-23 |
| e(y)3 | up | 0.9014 | 8.199e-23 |
| Trxr-1 | up | 1.139 | 1.335e-22 |
| Pa1 | up | 1.987 | 1.445e-22 |
| CG1943 | up | 1.14 | 1.445e-22 |
| PEK | up | 1.254 | 5.637e-22 |
| GstD1 | up | 0.8593 | 7.088e-22 |
| phol | up | 1.028 | 8.707e-22 |
| CG3680 | up | 0.8435 | 4.633e-21 |
| TfIIEalpha | up | 1.076 | 1.121e-20 |
| da | up | 0.8578 | 2.725e-20 |
| cact | up | 0.8173 | 3.294e-20 |
| Ran | up | 0.7748 | 6.905e-20 |
| CG1677 | up | 0.8251 | 9.427e-20 |
| betaTub56D | up | 1.038 | 1.749e-19 |
| Cctgamma | up | 0.8277 | 6.345e-19 |
| BRWD3 | up | 0.8852 | 7.197e-19 |
| l(1)G0469 | up | 1.272 | 7.197e-19 |
| Pdk | up | 1.35 | 1.224e-18 |
2.2 Cold shock
| gene | cold_fc | log2FoldChange.cold | padj.cold |
|---|---|---|---|
| stan | down | -2.535 | 2.296e-20 |
| Dhc93AB | down | -3.204 | 2.253e-19 |
| CG3339 | down | -4.294 | 5.99e-19 |
| CG1801 | down | -3.666 | 5.99e-19 |
| CG3280 | down | -3.068 | 5.99e-19 |
| CG42534 | down | -4.469 | 7.335e-19 |
| CG32791 | down | -1.855 | 1.687e-17 |
| ft | down | -2.987 | 8.646e-17 |
| pip | down | -2.89 | 1.435e-16 |
| Dscam1 | down | -1.683 | 2.136e-16 |
| CG43163 | down | -3.52 | 4.912e-16 |
| CG9492 | down | -3.725 | 5.06e-16 |
| rk | down | -3.238 | 8.245e-16 |
| Dhc36C | down | -2.296 | 1.463e-15 |
| CG8219 | down | -5.491 | 1.463e-15 |
| SIFaR | down | -4.039 | 2.331e-15 |
| Ac78C | down | -2.361 | 3.608e-15 |
| Ddr | down | -3.388 | 6.724e-15 |
| d | down | -3.775 | 1.027e-14 |
| CR45604 | down | -3.018 | 1.027e-14 |
| mtt | down | -3.664 | 1.135e-14 |
| Dhc62B | down | -3.892 | 1.49e-14 |
| iav | down | -4.075 | 1.696e-14 |
| CR45272 | down | -4.647 | 2.839e-14 |
| prom | down | -2.737 | 2.839e-14 |
| CG18519 | down | -3.233 | 2.839e-14 |
| CG43110 | down | -3.216 | 4.113e-14 |
| Nep5 | down | -3.804 | 4.113e-14 |
| conv | down | -3.69 | 6.626e-14 |
| rdgC | down | -1.94 | 6.946e-14 |
| Shawl | down | -3.097 | 7.672e-14 |
| CG44153 | down | -3.691 | 8.047e-14 |
| Hsp68 | down | -1.071 | 8.344e-14 |
| GluRIB | down | -2.415 | 9.254e-14 |
| CG45002 | down | -1.938 | 9.254e-14 |
| Sb | down | -3.89 | 1.557e-13 |
| Nlg4 | down | -2.28 | 1.557e-13 |
| CG4744 | down | -5.989 | 1.789e-13 |
| trh | down | -2.138 | 2.011e-13 |
| btv | down | -3.901 | 2.345e-13 |
| rdo | down | -3.167 | 2.827e-13 |
| Dop1R1 | down | -2.284 | 4.304e-13 |
| unc-13-4A | down | -2.109 | 4.464e-13 |
| TrpA1 | down | -4.111 | 4.79e-13 |
| CG42255 | down | -1.631 | 5.093e-13 |
| CG31814 | down | -3.185 | 5.781e-13 |
| rad | down | -1.922 | 6.059e-13 |
| Muc68Ca | down | -3.064 | 6.059e-13 |
| CG43795 | down | -2.427 | 6.591e-13 |
| eag | down | -1.562 | 7.105e-13 |
| Jafrac1 | up | 0.6228 | 1.102e-19 |
| Lsd-2 | up | 0.8045 | 1.486e-18 |
| endos | up | 0.7605 | 1.544e-17 |
| D1 | up | 0.684 | 1.687e-17 |
| Muc4B | up | 2.542 | 1.687e-17 |
| Capr | up | 0.7236 | 2.497e-16 |
| CG14478 | up | 0.8465 | 3.717e-15 |
| CG1677 | up | 0.73 | 7.404e-15 |
| Ran | up | 0.6814 | 7.51e-15 |
| CG15771 | up | 0.6845 | 1.537e-14 |
| bif | up | 0.7919 | 2.869e-14 |
| GstD1 | up | 0.7024 | 3.842e-14 |
| e(y)3 | up | 0.7182 | 4.174e-14 |
| mts | up | 0.638 | 6.428e-14 |
| phol | up | 0.8291 | 9.254e-14 |
| east | up | 0.7952 | 1.511e-13 |
| Act5C | up | 0.6184 | 2.345e-13 |
| CG8223 | up | 0.9441 | 2.752e-13 |
| cact | up | 0.6657 | 4.446e-13 |
| Rip11 | up | 0.7142 | 5.651e-13 |
| CG1440 | up | 0.6414 | 5.951e-13 |
| ssx | up | 0.6681 | 7.105e-13 |
| swa | up | 0.8273 | 1.017e-12 |
| tral | up | 0.8757 | 1.203e-12 |
| CG1943 | up | 0.8542 | 1.883e-12 |
| Pen | up | 0.898 | 2.887e-12 |
| cib | up | 0.8765 | 3.891e-12 |
| nocte | up | 0.7272 | 5.576e-12 |
| CG6597 | up | 0.6554 | 5.593e-12 |
| CG8963 | up | 0.6505 | 5.707e-12 |
| mim | up | 0.7948 | 6.11e-12 |
| Hmt4-20 | up | 0.7751 | 6.589e-12 |
| CG12262 | up | 0.8364 | 1.29e-11 |
| 26-29-p | up | 0.8999 | 1.457e-11 |
| rngo | up | 0.6756 | 1.476e-11 |
| Jupiter | up | 0.6516 | 1.526e-11 |
| CG4511 | up | 0.7374 | 1.68e-11 |
| da | up | 0.6498 | 1.814e-11 |
| pr-set7 | up | 0.8255 | 2.345e-11 |
| Cap | up | 0.775 | 2.683e-11 |
| CG10492 | up | 0.844 | 2.975e-11 |
| Gapdh2 | up | 0.7177 | 3.343e-11 |
| Rpt1 | up | 0.5432 | 3.942e-11 |
| Df31 | up | 0.7823 | 4.048e-11 |
| CG8108 | up | 0.6396 | 4.688e-11 |
| betaTub56D | up | 0.7807 | 5.6e-11 |
| Upf1 | up | 0.6236 | 5.655e-11 |
| CG9705 | up | 0.7375 | 8.723e-11 |
| Abi | up | 0.6488 | 8.733e-11 |
| Amun | up | 0.7009 | 9.284e-11 |
3 Shared and unique - LFC scatter
3.1 GWAS density plots
3.2 No labels
3.3 GWAS hits labeled
3.4 With unique labeled
3.5 With shared labeled
3.6 With shared labeled (padj.hot)
4 GWAS
4.1 CTmax
4.1.1 All hits (pval cutoff 10^-5)
| ID | gene | grps | AvgEff | AvgMixedPval |
|---|---|---|---|---|
| 2R_10330800_INS | Oaz | INTRON | 0.2164 | 8.00e-07 |
| 2R_6398772_SNP | lola | INTRON | 0.3719 | 9.00e-07 |
| 2R_6398743_SNP | lola | INTRON | 0.3710 | 9.00e-07 |
| 2R_10330805_SNP | Oaz | INTRON | 0.2332 | 1.00e-06 |
| 3R_22515826_SNP | 0.1838 | 2.30e-06 | ||
| 2L_12384183_SNP | bru-2 | INTRON | 0.1670 | 2.40e-06 |
| 3R_25373467_SNP | 0.3578 | 2.50e-06 | ||
| 2R_17564376_SNP | CG30263 | SYNONYMOUS_CODING | 0.1794 | 2.60e-06 |
| 2R_10330722_SNP | Oaz | INTRON | 0.2064 | 3.00e-06 |
| X_3381582_SNP | CG10803 | INTRON | 0.1864 | 3.20e-06 |
| X_3381590_SNP | CG10803 | INTRON | 0.1864 | 3.20e-06 |
| 2R_10330717_SNP | Oaz | INTRON | 0.2102 | 3.50e-06 |
| X_3381391_SNP | CG10803 | INTRON | 0.1804 | 3.70e-06 |
| X_3381587_SNP | CG10803 | INTRON | 0.1849 | 4.10e-06 |
| 3L_9575326_SNP | CG42673 | INTRON | 0.2063 | 6.00e-06 |
| X_5876026_SNP | Grip | INTRON | -0.1524 | 6.00e-06 |
| 3R_3427376_SNP | CG34127 | INTRON | 0.1614 | 7.60e-06 |
| 2L_19428598_SNP | CG16771 | NON_SYNONYMOUS_CODING | 0.1940 | 8.40e-06 |
| 2R_10330701_SNP | Oaz | INTRON | 0.2015 | 9.70e-06 |
| 2L_19428336_SNP | CG16771 | SYNONYMOUS_CODING | 0.2192 | 9.90e-06 |
| 3R_20025347_SNP | twin | INTRON | 0.1599 | 1.00e-05 |
| 3L_14419091_SNP | bbg | INTRON | -0.1660 | 1.03e-05 |
| 3L_1853643_SNP | CG13936 | INTRON | -0.1620 | 1.42e-05 |
| 3R_18941021_SNP | Rassf | INTRON | 0.1441 | 2.59e-05 |
| 2R_2894462_SNP | Tsp42Ec | INTRON | 0.1546 | 2.92e-05 |
| 3L_1853640_SNP | CG13936 | INTRON | -0.1510 | 3.62e-05 |
| 3R_7950242_SNP | CG4702 | INTRON | 0.1614 | 3.67e-05 |
| 3R_23411999_SNP | raps | UTR_3_PRIME | 0.3155 | 4.13e-05 |
| X_15304333_SNP | cngl | INTRON | 0.3127 | 4.89e-05 |
4.1.2 Group count
| grps | n |
|---|---|
| INTRON | 23 |
| SYNONYMOUS_CODING | 2 |
| NON_SYNONYMOUS_CODING | 1 |
| UTR_3_PRIME | 1 |
4.1.3 Gene count (>2)
| gene | n |
|---|---|
| Oaz | 5 |
| CG10803 | 4 |
| CG13936 | 2 |
| CG16771 | 2 |
| lola | 2 |
4.2 CTmin
4.2.1 All hits (pval cutoff 10^-5)
| ID | gene | grps | AvgEff | AvgMixedPval |
|---|---|---|---|---|
| 2R_20313579_SNP | prom | INTRON | 0.7260 | 0.00e+00 |
| X_4385442_SNP | CG32773 | INTRON | 1.0300 | 1.00e-07 |
| 3R_13900959_SNP | 0.6348 | 2.00e-07 | ||
| 2R_20308502_SNP | prom | INTRON | 0.6173 | 4.00e-07 |
| 3R_12992895_SNP | Mur89F | INTRON | -0.6868 | 7.00e-07 |
| 2R_20313917_SNP | prom | INTRON | 0.6276 | 9.00e-07 |
| 2R_20313922_DEL | prom | INTRON | 0.6276 | 9.00e-07 |
| 2R_20313930_SNP | prom | INTRON | 0.6276 | 9.00e-07 |
| 2R_20313933_SNP | prom | INTRON | 0.6276 | 9.00e-07 |
| 2R_20313935_INS | prom | INTRON | 0.6276 | 9.00e-07 |
| 2R_20313938_SNP | prom | INTRON | 0.6276 | 9.00e-07 |
| 2R_20313940_MNP | prom | INTRON | 0.6276 | 9.00e-07 |
| 2R_20308558_SNP | prom | INTRON | 0.6119 | 1.00e-06 |
| 2R_20250118_SNP | bs | INTRON | 0.5472 | 1.00e-06 |
| 2R_20301097_SNP | CG3419 | SYNONYMOUS_CODING | 0.5562 | 1.10e-06 |
| 2R_20301239_SNP | CG3419 | NON_SYNONYMOUS_CODING | 0.5434 | 1.10e-06 |
| 2R_20308533_SNP | prom | INTRON | 0.5994 | 1.20e-06 |
| 2R_20313914_SNP | prom | INTRON | 0.6215 | 1.30e-06 |
| 2L_2612712_SNP | -0.7705 | 1.40e-06 | ||
| 2R_20313927_SNP | prom | INTRON | 0.6177 | 1.50e-06 |
| 2R_20313911_SNP | prom | INTRON | 0.6056 | 1.80e-06 |
| 2R_20307893_SNP | prom | NON_SYNONYMOUS_CODING | 0.5823 | 1.80e-06 |
| 2R_20313916_SNP | prom | INTRON | 0.6222 | 1.90e-06 |
| 2R_20308565_SNP | prom | INTRON | 0.6001 | 1.90e-06 |
| 2R_20309964_SNP | prom | INTRON | 0.6214 | 2.00e-06 |
| 2R_20309106_SNP | prom | INTRON | 0.5709 | 2.20e-06 |
| 3R_11364111_SNP | CG18519 | SYNONYMOUS_CODING | 0.6916 | 2.20e-06 |
| 2R_20313919_SNP | prom | INTRON | 0.6250 | 2.40e-06 |
| 3R_14850882_SNP | CG14285 | NON_SYNONYMOUS_CODING | 0.7111 | 3.10e-06 |
| 2R_20313912_SNP | prom | INTRON | 0.6180 | 3.20e-06 |
| 2L_3465546_SNP | msl-2 | NON_SYNONYMOUS_CODING | 0.9493 | 3.20e-06 |
| 2R_20301123_SNP | CG3419 | NON_SYNONYMOUS_CODING | 0.5247 | 3.30e-06 |
| 3R_13552400_SNP | Lgr1 | INTRON | -0.9664 | 3.60e-06 |
| 3R_11367299_SNP | CG18519 | NON_SYNONYMOUS_CODING | 0.7277 | 4.00e-06 |
| 3R_11288771_SNP | CG14869 | INTRON | 0.9176 | 4.20e-06 |
| 2R_20301091_SNP | CG3419 | SYNONYMOUS_CODING | 0.5258 | 4.20e-06 |
| 2R_12301745_SNP | Sema-2b | INTRON | -0.5963 | 4.20e-06 |
| 3R_12990694_SNP | Mur89F | INTRON | 0.5249 | 4.30e-06 |
| 2R_20309307_SNP | prom | INTRON | 0.5642 | 4.80e-06 |
| 3R_9949217_SNP | twf | SYNONYMOUS_CODING | 0.7970 | 5.60e-06 |
| 3R_13002842_SNP | Mur89F | INTRON | 0.5265 | 5.70e-06 |
| 2R_20309122_SNP | prom | INTRON | 0.5473 | 5.80e-06 |
| 3R_12997215_SNP | Mur89F | INTRON | 0.5159 | 6.00e-06 |
| 2L_3396579_SNP | 0.9004 | 6.60e-06 | ||
| 2L_3396577_SNP | 0.8995 | 6.70e-06 | ||
| 3R_13552010_SNP | Lgr1 | INTRON | -1.0970 | 6.70e-06 |
| 3L_4694662_SNP | CG15876 | SYNONYMOUS_CODING | -0.5194 | 7.50e-06 |
| X_5639729_SNP | IntS6 | INTRON | 0.8784 | 7.50e-06 |
| 2L_2612282_INS | CG15395 | DOWNSTREAM | -0.7986 | 7.60e-06 |
| 2R_7024700_SNP | BBS4 | SYNONYMOUS_CODING | 0.8879 | 7.80e-06 |
| 2R_20313809_SNP | prom | INTRON | 0.5613 | 9.00e-06 |
| 3L_5853164_SNP | 0.5565 | 9.60e-06 | ||
| 2L_3885863_SNP | capu | INTRON | 0.4909 | 9.70e-06 |
| 2R_20263633_SNP | bs | UTR_3_PRIME | 0.5679 | 1.07e-05 |
| 3L_8696154_SNP | 0.8089 | 1.07e-05 | ||
| 2R_20308557_SNP | prom | INTRON | 0.5645 | 1.08e-05 |
| 2R_20313929_SNP | prom | INTRON | 0.5872 | 1.11e-05 |
| 3R_11388164_SNP | 0.5080 | 1.22e-05 | ||
| 2R_15691303_SNP | -0.8674 | 1.23e-05 | ||
| 2R_20314643_SNP | prom | INTRON | 0.5688 | 1.26e-05 |
| 3L_14188075_SNP | nuf | INTRON | 0.5235 | 1.30e-05 |
| 2R_20250001_SNP | bs | INTRON | 0.5296 | 1.37e-05 |
| 2R_10963282_SNP | 0.5813 | 1.40e-05 | ||
| 3R_5901971_SNP | CG5361 | UPSTREAM | -0.9199 | 1.48e-05 |
| 2R_20308551_SNP | prom | INTRON | 0.5059 | 1.51e-05 |
| 2R_20306042_SNP | prom | INTRON | 0.4978 | 1.52e-05 |
| 2R_17037739_INS | CG30389 | INTRON | 0.9023 | 1.55e-05 |
| X_6270410_SNP | 0.6623 | 1.55e-05 | ||
| 3R_12992188_SNP | Mur89F | INTRON | 0.4925 | 1.61e-05 |
| 2R_20280640_SNP | slik | DOWNSTREAM | 0.5158 | 1.81e-05 |
| X_14991195_SNP | rab3-GEF | INTRON | 0.4949 | 1.83e-05 |
| 3R_12997318_SNP | Mur89F | INTRON | 0.4879 | 2.01e-05 |
| 2R_20263506_SNP | bs | NON_SYNONYMOUS_CODING | 0.5286 | 2.02e-05 |
| 2R_20263738_SNP | bs | UTR_3_PRIME | 0.5286 | 2.02e-05 |
| 2R_20263740_INS | bs | UTR_3_PRIME | 0.5286 | 2.02e-05 |
| 2R_20263742_SNP | bs | UTR_3_PRIME | 0.5286 | 2.02e-05 |
| 2R_3772050_SNP | Dgk | INTRON | 0.5197 | 2.10e-05 |
| 2R_20313932_SNP | prom | INTRON | 0.5951 | 2.19e-05 |
| 2R_20257701_SNP | bs | INTRON | 0.5248 | 2.30e-05 |
| 2R_20252998_SNP | bs | INTRON | 0.5041 | 2.36e-05 |
| 2L_16629322_SNP | CG42389 | INTRON | -0.4818 | 2.42e-05 |
| 2R_20280846_SNP | slik | DOWNSTREAM | 0.5190 | 2.43e-05 |
| X_5908964_SNP | 1.0450 | 2.58e-05 | ||
| 2R_20257613_SNP | bs | INTRON | 0.5202 | 2.70e-05 |
| 3R_5905483_SNP | -1.0390 | 2.70e-05 | ||
| 3R_4247244_SNP | CG43462 | INTRON | -0.5526 | 2.83e-05 |
| 3R_9755188_SNP | -0.5000 | 2.85e-05 | ||
| 2L_1964167_SNP | erm | INTRON | -0.6578 | 2.87e-05 |
| 2R_20280809_SNP | slik | DOWNSTREAM | 0.5103 | 2.96e-05 |
| X_7604829_INS | 1.0340 | 3.04e-05 | ||
| 2R_20260408_SNP | bs | INTRON | 0.5402 | 3.32e-05 |
| 2R_12696827_SNP | 0.5654 | 3.35e-05 | ||
| 2R_14565483_DEL | CG15086 | DOWNSTREAM | -1.0320 | 3.45e-05 |
| 2R_1991490_SNP | EcR | INTRON | 0.9278 | 3.60e-05 |
| 2R_1991491_SNP | EcR | INTRON | 0.9278 | 3.60e-05 |
| 2L_1090401_SNP | CG4629 | INTRON | -0.9396 | 3.60e-05 |
| 2R_17983519_SNP | CG11073 | INTRON | 0.4746 | 3.78e-05 |
| 3L_3812775_SNP | CG12006 | SYNONYMOUS_CODING | -0.4898 | 3.86e-05 |
| 2R_20258747_SNP | bs | INTRON | 0.5115 | 4.07e-05 |
| 3L_3812970_SNP | CG12006 | SYNONYMOUS_CODING | -0.6748 | 4.94e-05 |
4.2.2 Group count
| grps | n |
|---|---|
| INTRON | 60 |
| SYNONYMOUS_CODING | 8 |
| NON_SYNONYMOUS_CODING | 7 |
| DOWNSTREAM | 5 |
| UTR_3_PRIME | 4 |
| UPSTREAM | 1 |
4.2.3 Gene count (>2)
| gene | n |
|---|---|
| prom | 30 |
| bs | 12 |
| Mur89F | 6 |
| CG3419 | 4 |
| slik | 3 |
| CG12006 | 2 |
| CG18519 | 2 |
| EcR | 2 |
| Lgr1 | 2 |
5 DEG & GWAS correlation plots
5.1 Expression Plots
Cold vs Ctrl
Hot vs Ctrl
5.2 CTmax & DEG Corr
5.3 CTmin & DEG Corr
